INTRODUCTION

Section 2 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

Background

Lymph nodes, in conjunction with the spleen, tonsils, adenoids, and Peyer patches, are highly organized centers of immune cells that filter antigen from the extracellular fluid. Directly interior to the fibrous capsule is the subcapsular sinus. This allows lymph, an ultrafiltrate of blood, to traverse from the afferent lymph vessels, through the sinuses, and out the efferent vessels. The sinuses are studded with macrophages, which remove 99% of all delivered antigens.

Interior to the subcapsular sinus is the cortex, which contains primary follicles, secondary follicles, and the interfollicular zone. Follicles within the cortex are major sites of B-cell proliferation, whereas the interfollicular zone is the site of antigen-dependent T-cell differentiation and proliferation. The deepest structure within the lymph node is the medulla, consisting of cords of plasma cells and small B lymphocytes that facilitate immunoglobulin secretion into the exiting lymph.

The lymph node, with its high concentration of lymphocytes and antigen-presenting cells, is an ideal organ for receiving antigens that gain access through the skin or gastrointestinal tract. Nodes have considerable capacity for growth and change. Lymph node size depends on the person's age, the location of the lymph node in the body, and antecedent immunological events. In neonates, lymph nodes are barely perceptible, but a progressive increase in total lymph node mass is observed until later childhood. Lymph node atrophy begins during adolescence and continues through later life.

Pathophysiology

Most lymphadenopathy in children is due to benign self-limited disease such as viral infections, and adenopathy is secondary to an increase in normal lymphocytes and macrophages in response to an antigen. Other less common mechanisms responsible for adenopathy include nodal accumulation of inflammatory cells in response to an infection in the node (lymphadenitis), neoplastic lymphocytes or macrophages (lymphoma), or metabolite-laden macrophages in storage diseases (Gaucher disease).

Frequency

United States

The precise incidence of lymphadenopathy is not known, but estimates of palpable adenopathy in childhood vary from 38-45%,¹ and lymphadenopathy is one of the most common clinical problems encountered in pediatrics. Determining whether adenopathy is simply a normal response to frequent viral infections within an age group or if it is significant enough to consider more serious underlying disease is often difficult. In the United States, common viral and bacterial infections are overwhelmingly the most common cause of adenopathy. Infectious mononucleosis and cytomegalovirus (CMV) are important etiologies, but adenopathy is usually caused by common viral upper respiratory tract infections. Localized lymphadenitis is most often caused by staphylococci and beta-hemolytic streptococci. Other infections, such as human immunodeficiency virus (HIV), malignancies, and autoimmune diseases, are less common causes of adenopathy.

International

Infections that are rarely observed in the United States, such as tuberculosis, typhoid fever, leishmaniasis, trypanosomiasis, schistosomiasis, filariasis, and fungal infections, are common causes of lymphadenopathy in developing nations.² HIV infections must be strongly considered in areas of high incidence.

Mortality/Morbidity

In the United States, mortality and serious morbidity caused by adenopathy are unusual given the common infectious etiologies.

• HIV is an uncommon cause of adenopathy in the United States, but its associated mortality requires consideration.
• Malignancies, such as leukemia, lymphomas, and neuroblastoma, are the primary causes of mortality in the United States.
• Significant morbidity and mortality are also associated with rare serious infections, autoimmune disorders (eg, juvenile rheumatoid arthritis, systemic lupus erythematosus), histiocytoses, and storage diseases.

Race

Race is not a factor in most lymphadenopathy. Rare causes may be associated with particular ethnic groups (eg, sarcoidosis in Africans, Kikuchi-Fujimori disease in Asians).

Sex

Sex is not a factor associated with childhood lymphadenopathy.

Age

• Adenopathy is most common in young children whose immune systems are responding to newly encountered infections.
• Adenopathy may be seen in one third of neonates and infants, usually in nodes that drain areas with mild skin irritation. Generalized adenopathy is rare in the neonate but may be seen with congenital infections such as CMV.
• Adenopathy related to malignancy is rare at all ages but is more likely to be secondary to leukemia or neuroblastoma in younger children and to Hodgkin disease in adolescents.

CLINICAL

Section 3 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

History

• The differential diagnosis of lymphadenopathy is broad. A patient's medical history and review of systems can be important in narrowing this differential. Although the underlying etiology is often simple, self-limited infection, more serious underlying etiologies must be quickly recognized. Serious infections and malignancies are important considerations. Many of these issues are listed in the Outline - Etiologies of Lymphadenopathy.
• In adolescents, a history of intravenous drug use and sexual history should be elicited. Knowledge of the pattern of lymph drainage aids in evaluating regions upstream for the presence of an infectious focus.

Physical

• Assessing the size, location, and character of the adenopathy, along with any associated physical findings, is important. 
• Most children have palpable lymph nodes in the anterior cervical, inguinal, and axillary regions that, if evaluated by adult standards, would qualify as lymphadenopathy. Lymphoid mass increases steadily after birth until age 8-12 years, and undergoes progressive atrophy during puberty. In young children, anterior cervical lymph nodes as large as 2 cm, axillary nodes as large as 1 cm, and inguinal nodes as large as 1.5 cm in diameter are normal, and further evaluation is usually not indicated. In a series of 457 children, malignancy was usually associated with nodes larger than 3 cm in diameter.³ However, the presence of even shotty (<0.5 cm) supraclavicular or epitrochlear adenopathy may be associated with malignancy and warrants further evaluation. Newborns usually have small adenopathy (<0.5 cm), and larger nodes not associated with a focus of inflammation are an indication for further evaluation.
• Nodes with erythema, tenderness, warmth, and fluctuance suggest lymphadenitis. Nodes that are matted together, firm, and nontender suggest malignancy, although benign adenopathy may also be nontender or firm.
• Seek a focus of infection or inflammation in the territory drained by the lymph nodes. For example, the classic manifestation of group A streptococcal pharyngitis is sore throat, fever, and anterior cervical lymphadenopathy (tonsillar node). When examining the oropharynx, pay special attention to the dentition. Similarly, impetigo of the buttock area is associated with inguinal adenopathy. Scalp lesions, such as seborrheic dermatitis (cradle cap), can cause newborn occipital adenopathy.
• Consider the possibility that palpable "lymph nodes" may in fact be other masses; for example, branchial cysts and other benign tumors can mimic cervical adenopathy.⁴  
• A careful history and physical examination, with a consideration of the factors listed above, help determine whether an enlarged lymph node merits further investigation.

Causes

Generalized lymphadenopathy is defined as enlargement of more than 2 noncontiguous lymph node groups. A thorough history and physical examination are critical in establishing a diagnosis. Causes of generalized lymphadenopathy include infections, autoimmune diseases, malignancies, histiocytoses, storage diseases, benign hyperplasia, and drug reactions.

• Infections: Generalized lymphadenopathy is most often associated with systemic viral infections. Infectious mononucleosis results in widespread adenopathy. Roseola infantum (caused by human herpes virus 6), CMV, varicella, and adenovirus all cause generalized lymphadenopathy. HIV is often associated with generalized adenopathy, which may be the presenting sign. Although usually associated with localized node enlargement, some bacterial infections present with generalized adenopathy. Examples include typhoid fever caused by Salmonella typhi, syphilis, plague, and tuberculosis. Less common bacteremias, including those caused by endocarditis, result in generalized lymphadenopathies.
• Malignant etiologies
• • Concern about malignant etiologies often drives further diagnostic testing in children with adenopathy. Malignancy is often associated with constitutional signs, such as fever, anorexia, nonspecific aches and pains, weight loss, and night sweats. The acute leukemias and lymphomas often present with these nonspecific findings.
  • Generalized lymphadenopathy is present at diagnosis in two thirds of children with acute lymphoblastic leukemia (ALL) and in one third of children with acute myeloblastic leukemia (AML). Abnormalities of peripheral blood counts usually lead to the correct diagnosis. The lymphomas more often present with regional lymphadenopathy, but generalized lymphadenopathy occurs.
  • Constitutional signs and symptoms observed in the leukemias are less reliable findings in the lymphomas. Only one third of children with Hodgkin disease and 10% with non-Hodgkin lymphoma display them. Malignancies usually present with nodes that tend to be firmer and less mobile or matted; however, this finding can be misleading. Benign reactive lymph nodes may be associated with fibrotic reactions that make them firm.
• Storage diseases: Generalized lymphadenopathy is an important manifestation of the lipid storage diseases. In Niemann-Pick disease, sphingomyelin and other lipids accumulate in the spleen, liver, lymph nodes, and central nervous system. In Gaucher disease, the accumulation of the glucosylceramide leads to the engorgement of the spleen, lymph nodes, and the bone marrow. Although widespread lymphadenopathy is common, additional findings, such as hepatosplenomegaly and developmental delay in Niemann-Pick disease and blood dyscrasias in Gaucher disease, are usually present. These diagnoses are established by leukocyte assay.
• Drug reactions: Adverse drug reactions can cause generalized lymphadenopathy. Within a couple of weeks of initiating phenytoin, some patients experience a syndrome of regional or generalized lymph node enlargement, followed by a severe maculopapular rash, fever, hepatosplenomegaly, jaundice, and anemia. These symptoms abate 2-3 months after discontinuation of the drug. Several other drugs are implicated in similar symptomatology, including mephenytoin, pyrimethamine, phenylbutazone, allopurinol, and isoniazid.
• Other nonneoplastic etiologies: Rare nonneoplastic causes of generalized lymphadenopathy include Langerhans cell histiocytosis and Epstein-Barr virus (EBV)-associated lymphoproliferative disease. Autoimmune etiologies include juvenile rheumatoid arthritis, which often presents with adenopathy, especially during the acute phases of the disease. Sarcoidosis and graft verses host disease also merit consideration.

Regional lymphadenopathy involves enlargement of a single node or multiple contiguous nodal regions. Lymph nodes are clustered in groups throughout the body and are concentrated in the head and neck, axillae, mediastinum, abdomen, and along the vascular trunks of the extremities. Each group drains lymph from a particular region of the body. Knowledge of the pattern of lymph drainage aids in determining the etiology.

• Cervical lymphadenopathy: Cervical nodes drain the tongue, external ear, parotid gland, and deeper structures of the neck, including the larynx, thyroid, and trachea. Inflammation or direct infection of these areas causes subsequent engorgement and hyperplasia of their respective node groups. Adenopathy is most common in cervical nodes in children and is usually related to infectious etiologies. Lymphadenopathy posterior to the sternocleidomastoid is typically a more ominous finding, with a higher risk of serious underlying disease.
• • Infectious etiologies
  • • Cervical adenopathy is a common feature of many viral infections. Infectious mononucleosis often manifests with posterior and anterior cervical adenopathy. Firm tender nodes that are not warm or erythematous characterize this lymph node enlargement. Other viral causes of cervical lymphadenopathy include adenovirus, herpesvirus, coxsackievirus, and CMV. In herpes gingivostomatitis, impressive submandibular and submental adenopathy reflects the amount of oral involvement.
    • Bacterial infections cause cervical adenopathy by causing the draining nodes to respond to local infection or by the infection localizing within the node itself as a lymphadenitis. Bacterial infection often results in enlarged lymph nodes that are warm, erythematous, and tender. Localized cervical lymphadenitis typically begins as enlarged, tender, and then fluctuant nodes. The appropriate management of a suppurative lymph node includes both antibiotics and incision and drainage. Antibiotic therapy should always include coverage for Staphylococcus aureus and Streptococcus pyogenes.
    • In patients with cervical adenopathy, determine whether the patient has had recent or ongoing sore throat or ear pain. Examine the oropharynx, paying special attention to the posterior pharynx and the dentition. The classic manifestation of group A streptococcal pharyngitis is sore throat, fever, and anterior cervical lymphadenopathy. Other streptococcal infections causing cervical adenopathy include otitis media, impetigo, and cellulitis.
    • Atypical mycobacteria cause subacute cervical lymphadenitis, with nodes that are large and indurated but not tender. The only definitive cure is removal of the infected node.
    • Mycobacterium tuberculosis may manifest with a suppurative lymph node identical to that of atypical mycobacterium. Intradermal skin testing may be equivocal. A biopsy may be necessary to establish the diagnosis.
    • Catscratch disease, caused by Bartonella henselae, presents with subacute lymphadenopathy often in the cervical region. The disease develops after the infected pet (usually a kitten) inoculates the host, usually through a scratch. Approximately 30 days later, fever, headache, and malaise develop, along with adenopathy that is often tender. Several lymph node chains may be involved. Suppurative adenopathy occurs in 10-35% of patients. Antibiotic therapy has not been shown to shorten the course.
  • Noninfectious etiologies
  • • Malignant childhood tumors develop in the head and neck region in one quarter of cases. In the first 6 years of life, neuroblastoma, leukemia, non-Hodgkin lymphoma, and rhabdomyosarcoma (in order of decreasing frequency) are most common in the head and neck region. In children older than 6 years, Hodgkin disease and non-Hodgkin lymphoma both predominate. Children with Hodgkin disease present with cervical adenopathy in 80-90% of cases as opposed to 40% of those with non-Hodgkin lymphoma.
    • Kawasaki disease is an important cause of cervical adenopathy. These children have fever for at least 5 days, and cervical lymphadenopathy is one of the 5 diagnostic criteria (of which 4 are necessary to establish the diagnosis).
• Submaxillary and submental lymphadenopathy: These nodes drain the teeth, tongue, gums, and buccal mucosa. Their enlargement is usually the result of localized infection, such as pharyngitis, herpetic gingivostomatitis, and dental abscess.
• Occipital lymphadenopathy: Occipital nodes drain the posterior scalp. These nodes are palpable in 5% of healthy children. Common etiologies of occipital lymphadenopathy include tinea capitis, seborrheic dermatitis, insect bites, orbital cellulitis, and pediculosis. Viral etiologies include rubella and roseola infantum. Rarely, occipital lymphadenopathy may be noted after enucleation of the eye for retinoblastoma.
• Preauricular lymphadenopathy: Preauricular nodes drain the conjunctivae, skin of the cheek, eyelids, and temporal region of the scalp and rarely are palpable in healthy children. The oculoglandular syndrome consists of severe conjunctivitis, corneal ulceration, eyelid edema, and ipsilateral preauricular lymphadenopathy. Chlamydia trachomatis and adenovirus can cause this syndrome.
• Mediastinal lymphadenopathy
• • Mediastinal nodes drain the thoracic viscera, including the lungs, heart, thymus, and thoracic esophagus. Because these nodes are not directly demonstrable upon physical examination, their enlargement must be indirectly assessed. Supraclavicular adenopathy is often associated with mediastinal adenopathy. Mediastinal nodes may cause cough, wheezing, dysphagia, airway erosion with hemoptysis, atelectasis, and the obstruction of the great vessels, which constitutes superior vena cava syndrome. Airway compromise may be life threatening.
  • Mediastinal lymphadenopathy is usually a sign of serious underlying disease. More than 95% of mediastinal masses are caused by tumors or cysts. Lymphomas and acute lymphoblastic leukemia are the most common etiologies and usually involve the anterior mediastinum. These malignancies are associated with a high risk of superior vena cava syndrome and are associated with several potentially life-threatening complications, as follows:
  • • The danger of sedation of patients, especially in the supine position for scans and procedures (The prone position actually may be safer.)
    • The risk during intubation of these patients, usually at the time of biopsy or placement of a central venous catheter
    • The risk of cardiovascular collapse during general anesthesia because of compression of venous return or because of previously undiagnosed pleural effusions
    • The risk of losing the ability to establish a pathologic diagnosis because of the use of steroids or radiation therapy
  • Unlike most other adenopathies, mediastinal lymphadenopathy is less frequently a result of infection. Infections frequently involve the hilar region and include histoplasmosis, coccidioidomycosis, and tuberculosis.
  • Nonlymphoid mediastinal tumors may be confused with adenopathy. These include neurogenic tumors (usually found in the posterior mediastinum), germ cell tumors, and teratomas.
  • Nonneoplastic conditions may also be confused with mediastinal adenopathy. These include the typically large thymus of a child, substernal thyroid glands, bronchogenic cysts, and abnormalities of the great vessels.
• Supraclavicular lymphadenopathy
• • Supraclavicular nodes drain the head, neck, arms, superficial thorax, lungs, mediastinum, and abdomen. Left supraclavicular nodes also reflect intra-abdominal drainage and enlarge in response to malignancies in that region. This is particularly true when adenopathy in this region occurs in the absence of other cervical adenopathy.
  • Right supraclavicular nodes drain the lung and mediastinum and are typically enlarged with intrathoracic lesions.
  • Serious underlying disease is frequent in children with supraclavicular adenopathy and always merits further evaluation. The potential for malignancy necessitates peripheral blood counts, skin testing for tuberculosis, and chemical studies, including uric acid, lactate dehydrogenase, calcium (Ca), phosphorus (P), and renal and hepatic function studies. Chest radiography and possibly CT scanning are indicated.
  • Several important infections may occur with supraclavicular adenopathy, including tuberculosis, histoplasmosis, and coccidioidomycosis.
  • Early lymph node biopsy should be considered in children with supraclavicular adenopathy.
• Axillary lymphadenopathy
• • Axillary nodes drain the hand, arm, lateral chest, abdominal walls, and the lateral portion of the breast.
  • A common cause of axillary lymphadenopathy is catscratch disease. Local axillary skin infection and irritation commonly are associated with local adenopathy. Other etiologies include recent immunizations in the arm (particularly with bacille Calmette-Guerin vaccine), brucellosis, juvenile rheumatoid arthritis, and non-Hodgkin lymphoma.
  • Hidradenitis suppurativa is a condition of enlarged tender lymph nodes that typically affects children with obesity and is caused by recurrent abscesses of lymph nodes in the axillary chain. The etiology is unknown, and treatment may include antibiotics. Many patients require incision and drainage.
• Abdominal lymphadenopathy
• • Abdominal nodes drain the lower extremities, pelvis, and abdominal organs. Although abdominal adenopathy is not usually demonstrable upon physical examination, abdominal pain, backache, increased urinary frequency, constipation, and intestinal obstruction secondary to intussusception are possible presentations.
  • Mesenteric adenitis is thought to be viral in etiology and is characterized by right lower quadrant abdominal pain caused by nodal enlargement near the ileocecal valve. Differentiating mesenteric adenitis from appendicitis may be difficult.
  • Mesenteric adenopathy may be caused by non-Hodgkin lymphoma or Hodgkin disease.
  • Typhoid fever and ulcerative colitis are other etiologies of mesenteric adenopathy.
• Iliac and inguinal lymphadenopathy: The lower extremities, perineum, buttocks, genitalia, and lower abdominal wall drain to these nodes. They are typically palpable in healthy children, although they are usually no larger than 1-1.5 cm in diameter. Regional lymphadenopathy is typically caused by infection; however, insect bites and diaper dermatitis are also frequent. Nonlymphoid masses that may be confused with adenopathy include hernias, ectopic testes, and lipomas.

Outline - Etiologies of Lymphadenopathy

I. Generalized lymphadenopathy

1. Infections
2. 1. Viral
   2. • Common upper respiratory infections
      • Infectious mononucleosis
      • CMV
      • Acquired immunodeficiency syndrome
      • Rubella
      • Varicella
      • Measles
   3. Bacterial
   4. • Septicemia
      • Typhoid fever
      • Tuberculosis
      • Syphilis
      • Plague
   5. Protozoal - Toxoplasmosis
   6. Fungal - Coccidioidomycosis
3. Autoimmune disorders and hypersensitivity states
4. 1. Juvenile rheumatoid arthritis
   2. Systemic lupus erythematosus
   3. Drug reactions (eg, phenytoin, allopurinol)
   4. Serum sickness
5. Storage Diseases
6. 1. Gaucher disease
   2. Niemann-Pick disease
7. Neoplastic and proliferative disorders
8. 1. Acute leukemias
   2. Lymphomas (Hodgkin, non-Hodgkin)
   3. Neuroblastoma
   4. Histiocytoses

II. Regional lymphadenopathy

1. Cervical
2. 1. Viral upper respiratory infection
   2. Infectious mononucleosis
   3. Rubella
   4. Catscratch disease
   5. Streptococcal pharyngitis
   6. Acute bacterial lymphadenitis
   7. Toxoplasmosis
   8. Tuberculosis/atypical mycobacterial infection
   9. Acute leukemia
   10. Lymphoma
   11. Neuroblastoma
   12. Rhabdomyosarcoma
   13. Kawasaki disease
3. Submaxillary and submental
4. 1. Oral and dental infections
   2. Acute lymphadenitis
5. Occipital
6. 1. Pediculosis capitis
   2. Tinea capitis
   3. Secondary to local skin infection
   4. Rubella
   5. Roseola
7. Preauricular
8. 1. Local skin infection
   2. Chronic ophthalmic infection
   3. Catscratch disease
9. Mediastinal
10. 1. Acute lymphoblastic leukemia
    2. Lymphoma
    3. Sarcoidosis
    4. Cystic fibrosis
    5. Tuberculosis
    6. Histoplasmosis
    7. Coccidioidomycosis
11. Supraclavicular
12. 1. Lymphoma
    2. Tuberculosis
    3. Histoplasmosis
    4. Coccidioidomycosis
13. Axillary
14. 1. Local infection
    2. Catscratch disease
    3. Brucellosis
    4. Reactions to immunizations
    5. Lymphoma
    6. Juvenile rheumatoid arthritis
15. Abdominal
16. 1. Acute mesenteric adenitis
    2. Lymphoma
17. Inguinal
18. 1. Local infection
    2. Diaper dermatitis
    3. Insect bites
    4. Syphilis
    5. Lymphogranuloma venereum

DIFFERENTIALS

Section 4 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

Other Problems to be Considered

Insect bites
Roseola
Oral and dental infections
Typhoid fever
Upper respiratory infection
Acquired immunodeficiency syndrome (AIDS)

WORKUP

Section 5 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

Lab Studies

The laboratory evaluation must be directed by the history and physical examination and is based on the size and other characteristics of the nodes and the overall clinical assessment of the patient. When a laboratory evaluation is indicated, it must be driven by the clinical evaluation. The following studies are typically included:

• CBC count, including a careful evaluation of the peripheral blood smear. An erythrocyte sedimentation rate is nonspecific but may be helpful.
• Evaluation of hepatic and renal function and a urine analysis are useful to identify underlying systemic disorders that may be associated with lymphadenopathy. Additional studies, such as lactate dehydrogenase (LDH), uric acid, calcium, and phosphate, may be indicated if malignancy is suspected. Skin testing for tuberculosis is usually indicated.
• In evaluating specific regional adenopathy, lymph node aspirate for culture may be important if lymphadenitis is clinically suspected.
• Titers for specific microorganisms may be indicated, particularly if generalized adenopathy is present. These may include Epstein-Barr virus, CMV, B henselae, Toxoplasma species, and HIV.

Imaging Studies

• Chest radiography is usually the primary screening imaging study. Additional imaging studies are usually based on abnormal chest radiograph findings. Chest radiography is often helpful in elucidating mediastinal adenopathy and underlying diseases affecting the lungs, including tuberculosis, coccidioidomycosis, lymphomas, neuroblastoma, histiocytoses, and Gaucher disease.
• Supraclavicular adenopathy, with its high associated rate of serious underlying disease, may be an indication for other studies, including CT scanning of the chest, abdomen, or both.
• Nuclear medicine scanning is helpful in the evaluation of lymphomas.
• Ultrasonography may be helpful in evaluating the changes in the lymph nodes and in evaluating the extent of lymph node involvement in patients with lymphadenopathy.⁵
• In children with inguinal adenopathy or abdominal complaints, ultrasonography of the abdomen, CT scanning of the abdomen, or both may be indicated.

Procedures

• The critical question is often whether or not to perform a lymph node biopsy; this requires an overall assessment of the history and physical examination as described above. Treatment with antibiotics (covering the bacterial pathogens frequently implicated in lymphadenitis) followed by reevaluation in 2-4 weeks is reasonable if clinical findings suggest lymphadenitis. Benign reactive adenopathy may be safely observed for months.
• If the size, location, or character of the lymphadenopathy suggests malignancy, the need for laboratory studies and biopsy is more urgent. If laboratory testing is inconclusive, a lymph node biopsy is immediately indicated.
• Fine needle aspiration and core needle biopsy yield small samples with limited ability to perform flow cytometry and chromosomal analysis; most pediatric hematologists and pathologists prefer excisional biopsy.
• Excisional biopsy also has limitations and may yield a definitive diagnosis in only 40-60% of patients because of inadequate specimen size, improper handling, or node-sampling error. 
• Hodgkin disease may be associated with reactive changes in surrounding nodes, and sampling more accessible nodes may miss the underlying malignancy. 
• Sampling inguinal nodes may yield specimens with an architecture distorted by chronic inflammatory changes. 
• The surgeon should perform a biopsy on larger, firmer, and most recently enlarging nodes, even if it is technically difficult, with appropriate preparation and handling of the specimen. If an excisional biopsy does not reveal the diagnosis despite appropriate sampling practice, a second biopsy may be indicated if symptoms persist or worsen.

Histologic Findings

Histiologic findings depend on the underlying etiology of the lymphadenopathy. Nonspecific changes consistent with reactive adenopathy are often the only findings. This is helpful in ruling out malignancy, histiocytoses, granulomatous disorders, and storage diseases. Specific infections can be diagnosed if tissues are appropriately stained.

When examining the tissue, histiologic findings are often inadequate. Flow cytometric and chromosomal analysis may provide critical information to permit a diagnosis to be established.

Staging

Staging is relevant only when a specific malignancy is diagnosed as the etiology of lymphadenopathy.

TREATMENT

Section 6 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

Medical Care

Treatment is determined by the specific underlying etiology of lymphadenopathy.

• Most clinicians treat children with cervical lymphadenopathy conservatively. Antibiotics should be given only if a bacterial infection is suspected. This treatment is often given before biopsy or aspiration is performed. This practice may result in unnecessary prescription of antimicrobials. However, the risks of surgery often outweigh the potential benefits of a brief course of antibiotics. Most enlarged lymph nodes are caused by an infectious process. If aspects of the clinical picture suggest malignancy, such as persistent fevers or weight loss, biopsy should be pursued sooner.
• Management of superior vena cava syndrome requires emergency care, including chemotherapy and possibly radiation therapy.

Surgical Care

Surgical care usually involves a biopsy. If lymphadenitis is present, aspirate may be needed for culture, and removal of the affected node may be indicated.

Consultations

• Consultation with a pediatric hematologist, pediatric oncologist, or both is often useful, especially if the adenopathy seems to be more than reactive. Often, the most important decision for these children is whether further evaluation is necessary at all; experience in evaluating these children is frequently very helpful. The ability to provide a careful assessment of the peripheral blood smear may be particularly important.
• Surgical consultation is usually helpful for lymph node biopsy, needle aspiration for culture, and for incision and drainage of obviously infected fluctuant nodes.

Diet

• Diet plays little role in the pathophysiology of lymphadenopathy.
• Internationally, many of the infectious etiologies may be associated with a higher risk of malnutrition.

Activity

Limitations on activity usually involve associated acute-onset splenomegaly. Any patient with an acutely enlarged spleen may need to be restricted from contact sports. In infectious mononucleosis, rupture of the spleen can occur with relatively little trauma and can be fatal.

MEDICATION

Section 7 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

No specific medical therapy for lymphadenopathy is acknowledged. Therapy is directed at the specific diagnosis, once established, and when appropriate.

FOLLOW-UP

Section 8 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

Further Inpatient Care

• Additional inpatient treatment depends on establishing the diagnosis and determining management based on that diagnosis.

Further Outpatient Care

• Further outpatient treatment depends on establishing a diagnosis and determining management of that diagnosis.

In/Out Patient Meds

• Inpatient and ambulatory medications depend on the specific underlying etiology of the lymphadenopathy.

Transfer

• Transfer of the patient usually depends on the specific diagnosis. Patients who develop superior vena cava syndrome with either respiratory symptoms or obstruction to blood flow require emergency medical care and may require transfer to a tertiary care center.

Complications

• Complications are usually related to the specific underlying disorder causing the lymphadenopathy; however, the lymphadenopathy itself can cause potentially serious complications.
• Mediastinal adenopathy can result in several potentially life-threatening complications. Recognition of these complications is important because mediastinal adenopathy cannot be directly assessed clinically and therefore may be easily missed.
• Mediastinal adenopathy can cause superior vena cava syndrome with obstruction of blood flow; bronchial or tracheal obstruction with cough, wheezing, and ultimately respiratory tract obstruction (which can be life threatening); and dysphagia from esophageal compression. Occasionally, erosion of a node into a bronchus or trachea can result in hemoptysis.
• When the diagnosis of an underlying malignancy is missed, serious metabolic complications can occur. These include uric acid nephropathy, hyperkalemia, hypercalcemia, hypocalcemia, hyperphosphatemia, and acid renal failure.
• Abdominal adenopathy can cause abdominal or back pain, constipation, and urinary frequency. Intestinal obstruction caused by intussusception can be life threatening.

Prognosis

• The prognosis of lymphadenopathy almost entirely depends on the underlying etiology. Patients with specific complications, such as superior vena cava syndrome, are at risk unless this specific complication is managed. Their prognosis is dependent on the management of the neoplastic process resulting in superior vena cava syndrome.

Patient Education

• Patient and family education depends on the specific etiology of the lymphadenopathy.

MISCELLANEOUS

Section 9 of 10  

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

Medical/Legal Pitfalls

• Failure to diagnose an underlying malignancy is a major medical/legal issue. As with any decision about providing medical care, the risks of not making a timely diagnosis must be weighed against the potential benefits of treating an illness appropriately.
• Routine laboratory studies and certainly lymph node biopsies should not be obtained in most children who present with lymphadenopathy. Most of these children have self-limited illnesses that require no further treatment and no intervention. Otherwise, many children undergo unnecessary biopsy, with its cost, psychologic trauma, morbidity, and rare mortality.

REFERENCES

Section 10 of 10

• Authors and Editors
• Introduction
• Clinical
• Differentials
• Workup
• Treatment
• Medication
• Follow-up
• Miscellaneous
• References

1. Larsson LO, Bentzon MW, Berg Kelly K, et al. Palpable lymph nodes of the neck in Swedish schoolchildren. Acta Paediatr. Oct 1994;83(10):1091-4. [Medline].
2. Moore SW, Schneider JW, Schaaf HS. Diagnostic aspects of cervical lymphadenopathy in children in the developing world: a study of 1,877 surgical specimens. Pediatr Surg Int. Jun 2003;19(4):240-4. [Medline].
3. Oguz A, Karadeniz C, Temel EA, Citak EC, Okur FV. Evaluation of peripheral lymphadenopathy in children. Pediatr Hematol Oncol. Oct-Nov 2006;23(7):549-61. [Medline].
4. Yaris N, Cakir M, Sozen E, Cobanoglu U. Analysis of children with peripheral lymphadenopathy. Clin Pediatr (Phila). Jul 2006;45(6):544-9. [Medline].
5. Niedzielska G, Kotowski M, Niedzielski A, Dybiec E, Wieczorek P. Cervical lymphadenopathy in children--incidence and diagnostic management. Int J Pediatr Otorhinolaryngol. Jan 2007;71(1):51-6. [Medline].
6. Grossman M, Shiramizu B. Evaluation of lymphadenopathy in children. Curr Opin Pediatr. 1994;6(1):68-76. [Medline].
7. Kliegman RM, Nieder ML, Super DM. Lymphadenopathy. In: Fletcher J, Bralow L, eds. Practical Strategies in Pediatric Diagnosis and Therapy. WB Saunders Co; 1996:791-803.
8. Miller DR. Hematologic malignancies: leukemia and lymphoma (Differential diagnosis of lymphadenopathy). In: Miller DR, Baehner RL, eds. Blood Diseases of Infancy and Childhood. Mosby Inc; 1995:745-9.
9. Nield LS, Kamat D. Lymphadenopathy in children: when and how to evaluate. Clin Pediatr (Phila). Jan-Feb 2004;43(1):25-33. [Medline].
10. Roberts KB, Tunnessen WW. Lymphadenopathy. In: Signs and Symptoms in Pediatrics. 3^rd ed. Lippincott, Williams, and Wilkins; 1999:63-72.
11. Twist CJ, Link MP. Assessment of lymphadenopathy in children. Pediatr Clin North Am. Oct 2002;49(5):1009-25. [Medline].
12. Vayner N, Coret A, Polliack G, et al. Mesenteric lymphadenopathy in children examined by US for chronic and/or recurrent abdominal pain. Pediatr Radiol. Dec 2003;33(12):864-7. [Medline].

Article Last Updated: Nov 8, 2007